Mapping sequences required for nuclear localization and the transcriptional activation function of the Arabidopsis protein AINTEGUMENTA
Identifieur interne : 000993 ( Istex/Checkpoint ); précédent : 000992; suivant : 000994Mapping sequences required for nuclear localization and the transcriptional activation function of the Arabidopsis protein AINTEGUMENTA
Auteurs : Beth A. Krizek [États-Unis] ; Chidananda Sulli [États-Unis]Source :
- Planta [ 0032-0935 ] ; 2006-08-01.
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Abstract
Abstract: The Arabidopsis thaliana floral development protein AINTEGUMENTA (ANT) is a member of a large family of DNA binding proteins (AP2/ERF family) that control plant growth and development in response to developmental or environmental signals. Transcriptional activation and/or repression activities have been demonstrated for several members of this protein family. We have used fusions between ANT and the GAL4 DNA binding domain to identify an 80 amino acid sequence important for the transcriptional activation function of ANT. This region shows similarity to transcriptional activation domains in other proteins, as it is rich in Ser/Thr, Gln/Asn, and acidic amino acids. We also demonstrate that ANT can activate gene expression in Arabidopsis plants through binding to a DNA sequence corresponding to an in vitro determined ANT binding site. Finally, we show that ANT is nuclear localized and that the sequence KKKR (amino acids 252–255) is required for nuclear localization of the protein.
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DOI: 10.1007/s00425-006-0253-9
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Krizek</name><affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biological Sciences, University of South Carolina, 29208, Columbia, SC</wicri:regionArea><orgName type="university">Université de Caroline du Sud</orgName><placeName><settlement type="city">Columbia (Caroline du Sud)</settlement><region type="state">Caroline du Sud</region></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">États-Unis</country></affiliation></author><author><name sortKey="Sulli, Chidananda" sort="Sulli, Chidananda" uniqKey="Sulli C" first="Chidananda" last="Sulli">Chidananda Sulli</name><affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biological Sciences, University of South Carolina, 29208, Columbia, SC</wicri:regionArea><orgName type="university">Université de Caroline du Sud</orgName><placeName><settlement type="city">Columbia (Caroline du Sud)</settlement><region type="state">Caroline du Sud</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Planta</title><title level="j" type="sub">An International Journal of Plant Biology</title><title level="j" type="abbrev">Planta</title><idno type="ISSN">0032-0935</idno><idno type="eISSN">1432-2048</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="2006-08-01">2006-08-01</date><biblScope unit="volume">224</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="612">612</biblScope><biblScope unit="page" to="621">621</biblScope></imprint><idno type="ISSN">0032-0935</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0032-0935</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>AINTEGUMENTA</term><term>AP2/ERF family</term><term>Arabidopsis thaliana</term><term>Flower development</term><term>Nuclear localization</term><term>Transcriptional activation</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The Arabidopsis thaliana floral development protein AINTEGUMENTA (ANT) is a member of a large family of DNA binding proteins (AP2/ERF family) that control plant growth and development in response to developmental or environmental signals. Transcriptional activation and/or repression activities have been demonstrated for several members of this protein family. We have used fusions between ANT and the GAL4 DNA binding domain to identify an 80 amino acid sequence important for the transcriptional activation function of ANT. This region shows similarity to transcriptional activation domains in other proteins, as it is rich in Ser/Thr, Gln/Asn, and acidic amino acids. We also demonstrate that ANT can activate gene expression in Arabidopsis plants through binding to a DNA sequence corresponding to an in vitro determined ANT binding site. Finally, we show that ANT is nuclear localized and that the sequence KKKR (amino acids 252–255) is required for nuclear localization of the protein.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Caroline du Sud</li></region><settlement><li>Columbia (Caroline du Sud)</li></settlement><orgName><li>Université de Caroline du Sud</li></orgName></list><tree><country name="États-Unis"><region name="Caroline du Sud"><name sortKey="Krizek, Beth A" sort="Krizek, Beth A" uniqKey="Krizek B" first="Beth A." last="Krizek">Beth A. Krizek</name></region><name sortKey="Krizek, Beth A" sort="Krizek, Beth A" uniqKey="Krizek B" first="Beth A." last="Krizek">Beth A. Krizek</name><name sortKey="Sulli, Chidananda" sort="Sulli, Chidananda" uniqKey="Sulli C" first="Chidananda" last="Sulli">Chidananda Sulli</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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